Sequential role of RAD51 paralog complexes in replication fork ... - Nature
WEBJul 15, 2020 · siRNA-mediated depletion of the human proteins suggested different roles for the two RAD51 paralog subcomplexes at early (BCDX2) versus late (CX3) stages of HR-mediated DSB repair 43,44,45.
RAD51C-Deficient Cancer Cells Are Highly Sensitive to the PARP ...
WEBJun 9, 2013 · The siRNA-mediated knockdown of RAD51C increased the olaparib-induced apoptosis and G 2 –M cell-cycle arrest compared with the control siRNA transfection . The olaparib-induced apoptosis in RAD51C-depleted SNU-668 cells was also confirmed by detecting the PARP cleavage ( Fig. 2B ).
Clinical significance of RAD51C and its contribution to ovarian ...
WEBJan 1, 2020 · Transfection of siRNA-3 into A2780 significantly down-regulated RAD51C expression, which inhibited cellular proliferation and migration both in early and terminal stages of apoptosis, suggesting an important role of RAD51C during the carcinogenesis and progression of ovarian cancer.
The RAD51 recombinase protects mitotic chromatin in human …
WEBSep 10, 2021 · In this study, we show that RAD51 protects under-replicated DNA in mitotic human cells and, in this way, promotes mitotic DNA synthesis (MiDAS) and successful chromosome segregation. In cells...
Regulation of RAD51 at the Transcriptional and Functional Levels: …
WEBJun 11, 2021 · We review here the multiple levels of regulation of RAD51 expression and function and explore potential therapeutic leads. Abstract. The RAD51 recombinase is a critical effector of Homologous Recombination (HR), which is an essential DNA repair mechanism for double-strand breaks.
RAD-ical New Insights into RAD51 Regulation - PMC - National …
WEBDec 13, 2018 · For example, small interfering RNA (siRNA) depletion of any individual RAD51 paralog destabilizes its binding partners (i.e., siXRCC2 decreases the expression of endogenous RAD51D), and siRNAs have variable levels of knockdown efficiency [74,106].
WEBHere we report the use of RNA interference to deplete expression of Rad51C protein in human HT1080 and HeLa cells. In HT1080 cells, depletion of Rad51C by small interfering RNA caused a significant reduction of frequency in homolo-gous recombination.
Human Rad51C Deficiency Destabilizes XRCC3, Impairs Recombination…
WEBHere we report the use of RNA interference to deplete expression of Rad51C protein in human HT1080 and HeLa cells. In HT1080 cells, depletion of Rad51C by small interfering RNA caused a significant reduction of frequency in homologous recombination.
RAD51C-XRCC3 structure and cancer patient mutations define …
WEBJul 24, 2023 · Here we report the RAD51C-XRCC3 (CX3) X-ray co-crystal structure with bound ATP analog and define separable RAD51C replication stability roles informed by its three-dimensional structure,...
Interplay between human DNA repair proteins at a unique …
WEBJan 5, 2006 · DNA repair by homologous recombination is essential for preserving genomic integrity. The RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3) play important roles in this process. In this study, we show that human RAD51 interacts with RAD51C‐XRCC3 or RAD51B‐C‐D‐XRCC2.
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